In this experiment , radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process . methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0 . 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al . thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum , and added to 50ml cell culture flasks 方法:胰島的分離參照王玲等的方法,每次實驗取新生3 - 5天sd大鼠,無菌條件下剖腹取出胰腺,剪切為0 . 5mm ~ 3的組織塊, v型膠原酶消化30min后,離心洗滌,懸浮于完全培養(yǎng)基,接種入50ml培養(yǎng)瓶,于5 co _ 2 、 95空氣條件下培養(yǎng)20h ,轉(zhuǎn)板純化,接種于96孔培養(yǎng)板培養(yǎng)24h ,按實驗要求進(jìn)行實驗。